Examine the outside of the reagent bottle for any liquid that has dripped down the bottle or spilled on the counter top. Some laboratory methods are best studied by crosschecking with other sections to which the technique relates. Precipitation and water purity chemistry 540 physical organic chemistry except where otherwise noted, content on this site is licensed under a creative commons attribution 4. You will become familiar with general information about the use of lab equipment, as well as more detailed information about the stepbystep procedures for the specific instruments that you use. Forensic laboratory techniques 75 electron microscopes compound microscopes, stereomicroscopes, and comparison microscopes all use light. Preservation of laboratory samples, specimens, and reagents using extreme cold is routinely performed in biomedical research labs. June 2002 overview this microbiology laboratory manual is designed especially for the nonmajors, health science. There are chemicals in bottles, flame sources, and all kinds of fragile glassware. Laboratory techniques transfection use of viruses to get dna into cells in eukaryote and bacteria transduction figure 7. It is because glass is resistant to the action of most of the chemicals. Virology techniques introduction virology is a field within microbiology that encompasses the study of viruses and the diseases they cause.
Upon completion of the lecture, accompanying video, required readings and clinical rotations, the student will be able to. The organic lab manual provides detailed and comprehensive material of general organic laboratory techniques and instrumentation. Aseptic technique is a set of routine measures that are taken to prevent cultures, sterile media stocks, and other solutions from being contaminated by unwanted microorganisms i. Safety pdf safety and general laboratory procedures. Laboratory techniques relate to many other sections in this publication. Your practical guide to basic laboratory techniques science. An acidbase extraction is a type of liquidliquid extraction. For medical laboratory technology students introduction to medical laboratory technology. At the lab, forensic scientists may perform presumptive tests to determine what kind of body fluid. Always update books hourly, if not looking, search in the book search column. Jan 14, 2020 general aseptic techniques in microbiology laboratory. This section includes course modules for mastering a series of chemistry laboratory techniques. In this resource you will find theory and procedures on the main organic lab techniques chromatography, crystallization, extraction, distillation as well as general concepts on how to set up and heat apparatuses. Biology laboratory techniques wikibooks, open books for.
This lecture note is primarily prepared for medical laboratory technology students pursuing their studies at bachelorrate level in various higher teaching institutions. Pdf on may 1, 2017, mathew folaranmi olaniyan and others published lecture notes on laboratory instrumentation. Weighing, c measuring volume of a liquid, d evaporation and filtration students will get trained in the correct usage of these techniques. It typically involves different solubility levels in water and an organic solvent.
Part of the general chemistry section of the dat will ask you laboratory questions. Lab techniques are the processes and practices that are recommended for using the various equipment in the laboratory. Labs chemistry laboratory techniques chemistry mit. In this lesson, we will go over some of the most basic lab techniques you. Usually, they only comprise of 12 questions on test, and most are easy to answer. The type of activities a student will perform vary depending on the exact field they are in. Gain clinical exposure and practice of skills and theory learned in the previous clinical laboratory science courses in a general laboratory setting. Having pictures alongside the text is very helpful, and it could have been supplemented with links to videos if available.
Describe the general concept of aseptic techniques used in laboratory preparation and analysis explain and differentiate between sterilization, disinfection, and sanitization describe the different types of culture media and their possible physical state discuss inoculation, incubation, and isolation. An important part of working in any laboratory is the proper use and calibration of instruments and equipment. Who manual of basic techniques for a health laboratory. The laboratory apparatus for carrying out reactions, in general, is made up of glass. A sponsored supplement to science your practical guide to. Jove science education general laboratory techniques. The lab manual also compares different techniques, and highlights their use in specific cases, which is great if the students are involved in independent work or research. Fundamental techniques in cell culture sigmaaldrich. Chm151l will often reinforce lecture course topics, covering them at the same time or after, but the primary purpose is to provide you with instruction in common laboratory manipulations, laboratory safety. These microscopes can magnify materials up to 500,000. There are more than 1 million books that have been enjoyed by people from all over the world.
Welcome to the online depository for basic chemistry techniques. Ppt general laboratory technique powerpoint presentation. Nigerian institute of science laboratory technology. Identification of substances by physical properties relates to chap. Relate the demands and responsibilities and duties of the cls and other staff in a general service laboratory. The laboratory and lecture are separate courses and you will be assigned a separate grade for each. The microplate reader is a multimodal instrument that allows for a variety of experiments. Introduction to general chemistry i laboratory general chemistry i laboratory chm151l is designed to be taken by students enrolled in general chemistry i lecture chm151. Microbiological laboratory techniques clinical gate. The training programme covers core aspects of slt profession which include laboratory management and administration, laboratory instrument application, equipment maintenance, general laboratory techniques and procedures, professional ethics, glass blowing and. Safety in the laboratory iv common laboratory equipment ix experiment 1. The organic solvent may be any carbonbased liqiuid that does not dissolve very well in water.
Laboratory sessions are focused on pure culture techniques, methods of staining and the microscopic, colonial and biochemical identification of microorganisms. Laboratory techniques, as used in biology, biochemistry, chemistry, molecular biology, etc. The training programme covers core aspects of slt profession which include laboratory management and administration, laboratory instrument application, equipment maintenance, general laboratory techniques and procedures, professional ethics, glass blowing and project. Separation of the components of a mixture relates to chap.
You will become familiar with general information about the use of lab equipment, as well as more detailed information about the stepbystep procedures. Organic chemistry laboratory techniques open textbook. Get ebooks laboratory manual for principles of general chemistry on pdf, epub, tuebl, mobi and audiobook for free. Plant geneticist, for example, sometimes spend time out in fields gathering plants, while molecular biologists may use complex equipment such as dna sequencing. If so, a sample will be collected and sent to the lab. When you first set foot inside a chemistry lab, it can be a little overwhelming. Chm151l will often reinforce lecture course topics.
The manual is intended to be the cornerstone of a safety program designed to aid faculty, staff, and students in maintaining a safe environment in which to teach, conduct research, and learn. Grading policy pdf details of the experiment grade and the final grade for the course. Brown this collection of general chemistry laboratory techniques teaches the student how a lab functions within all scientific curriculums for all teaching methods, including inquiry based curriculums. This chapter addresses the control of the analytical process in the laboratory, as distinct from meeting the typical analytical needs of a specific project. Filtration processes can also be distinguished by general application. Mar 05, 2020 biology is a hands on science, and biology students are usually required to spend some time in the laboratory. Techniques manual dltm, used as supplementary material for this. Laboratory techniques for general chemistry, 1st edition.
Forensic laboratory techniques 73 uv ultraviolet light, or another alternative light source, to determine whether a stain is a body fluid. To link to this object, paste this link in email, im or document to embed this object, paste this html in website. Your teacher will show you the proper procedures for cleaning up a chemical spill. Centrifugation is the process by which a centrifuge. Home wayne steinmetz chemistry education resources common laboratory techniques and practices reference url. This category has the following 16 subcategories, out of 16 total. In modern equipment, an immersion heating circulator is mounted onto the rear panel of. Common laboratory techniques and practices wayne steinmetz.
General laboratory safety manual university of houston. The general laboratory is divided into these key areas a. The purpose of this lesson is to provide a general overview of laboratory techniques used in the. All procedures are accompanied by stepbystep pictures, and.
Jun 23, 2019 welcome to the online depository for basic chemistry techniques. The following standard and special practices, safety equipment and facilities apply to agents assigned to biosafety level 1. Plant geneticist, for example, sometimes spend time out in fields gathering plants, while molecular biologists may use complex equipment such. The handbook is intended as a guide rather than an indepth text book of cell culture and you are encouraged to consult relevant specialised literature to obtain more detailed information. Laboratory manual for principles of general chemistry pdf. Organic chemistry laboratory techniques open textbook library. All materials may be found in the complete laboratory manual. Quality is central to all laboratory methodologies. It is because glass is resistant to the action of most of the. In the laboratory, viruses have served as useful tools to better understand cellular mechanisms.
Laboratory techniques for general chemistry first edition 2012 stephen l. One of the most common applications for analytical filtration is sample preparation for hplc. This collection exhibits how to use standard pieces of laboratory equipment essential in many experiments. Biology laboratory techniques wikibooks, open books for an. Griffiths et al, 2000 laboratory techniques chromosome integration how to make stable mutants adds a gene to a chromosome homologous recombination. Bunsen burner used in the laboratory has two valves for adjustments and it is important that a burner is adjusted properly for best. Electron microscopes, on the other hand, use beams of electrons to form images. Information on the original research project assignment and a listing of techniques guides are also provided. It can also be helpful for those graduates who are in service. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext.
Due to the physical nature of the matrix, sample preparation for solids requires the most attention, and therefore is discussed at great length section 12. Methods of culturing microorganisms 3 five basic techniques 4 fig. Alberts et al, 2002 rotational symmetry laboratory techniques. It contains information about most instruments, and provides detailed explanation of their use. Laboratory manual and workbook in microbiology applications to patient care 7th edition josephine a. The development of, and adherence to, procedures in a general general laboratory. Generally, two types of glass are used for making apparatus. Biology is a hands on science, and biology students are usually required to spend some time in the laboratory. Wurtzel, department of biological sciences, lehman college very basic lab skills such as notebook keeping, fluid measurements, micropipettes, pipetting, using graduated cylinders, using microcentrifuge, sterile technique,plate streaking for single colonies and more. Where the scope of hazards is not adequately addressed by this general document, specific standard operating procedures must be developed by the principal. General laboratory technique 1 general laboratory technique 2 common apparatus equipment 3 common apparatus equipment 4 test tube and boiling tube 5 test tube holder 6 roundbottomed flask and flatbottomed flask stronger than the flatbottomed flask but clamping is required. The centrifuge is an instrument used in nearly every research lab across the globe.
In the development of this lecture note, materials have been gathered and adapted from different standard books. Hematology, urinalysis, coagulation, flow cytometry f. General aspects and guidelines that are common to many. Use this cheat sheet to study general chemistry lab techniques tested on the dat. Quality control provides quantitative estimates of analysis and measurement controls that can be used to determine compliance with project objectives. Access to the laboratory is limited or restricted at the discretion of. After reading this chapter, the student will be able to. Unit2 basic laboratory techniques t he laboratory apparatus for carrying out reactions, in general, is made up of glass.
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